KMID : 1101320130450010037
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Korean Journal of Clinical Laboratory Science 2013 Volume.45 No. 1 p.37 ~ p.42
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Cell Yield of Cerebrospinal Fluid Cell Count Using Cytocentrifuges
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Koo Bon-Kyung
Shim Hyun-Seol Oh Jung-A Lee Yong-Tag Choi Dae-Yong Lee Beom-Se Kim Eun-Jee Lee Seung-Tae Kim Sun-Hee
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Abstract
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The cells are concentrated approximately 20-fold by cytocentrifugation. This study evaluated the nucleated cell number for cells recovered on slide by using Cytospin-3 (Thermo Shandon Ltd. UK) and Cytopro-7620 (Wescor Inc., USA) cytocentrifuges to hematocytometer cell count of 0¡5 WBCs/¥ìL of hematocytometer in the cerebrospinal fluid cell count. One hundred forty eight samples of 0¡5 WBCs/¥ìL on hematocytometer, were cytocentrifuged by Cytospin-3 and Cytopro-7620 instruments. The nucleated cell number for cells recovered on slide was counted after Wright stain. The nucleated cell number for cells recovered on slide was 0¡40 cells in the 44 samples of 0 WBC/¥ìL, and 3¡95 cells in the 31 samples of 1 WBC/¥ìL. It was observed that the nucleated cell number for cells recovered on slide was 13¡100 cells in the 44 samples of 2 WBCs/¥ìL, and more than 100 cells in the 29 samples of 3¡5 WBCs/¥ìL, respectively. In addition, extremely normal lymphocyte, monocyte and polymorphonuclear neutrophil were observed in the 143 samples of 0¡5 WBCs/¥ìL. Macrophage and eosinophil were also rarely observed. The nucleated cell number for cells recovered on slide was 20 cells, which were regarded as 1 WBC/¥ìL in body fluid cell count. However, in this study, we made alterations to report nucleated cell percentage as 0% without preparing the cytocentrifuged slide at 0 WBC/¥ìL by using the cell yield in a comparison between the value of 0¡5 WBCs/¥ìL and nucleated cell number for cells recovered on slide.
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KEYWORD
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Cell yield, Cerebrospinal fluid, Cell count, Cytocentrifugation
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